Exercise Training Decreases Adipose Tissue Inflammation in Cachectic Rats

F. S. Lira; A. S. Yamashita; J. C. Rosa; C. H. Koyama; E. C. Caperuto; M. L. Batista Jr; M.C. L. Seelaender

doi:10.1055/s-0031-1299694

Hormone and Metabolic Research, Table of Contents

Horm Metab Res 2012; 44(02): 91-98
DOI: 10.1055/s-0031-1299694

Original Basic

Exercise Training Decreases Adipose Tissue Inflammation in Cachectic Rats

Authors

F. S. Lira

¹Cancer Metabolism Research Group, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil

²Department of Physiology, Division of Nutrition Physiology, Federal University of São Paulo, São Paulo, Brazil
A. S. Yamashita

¹Cancer Metabolism Research Group, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
J. C. Rosa

²Department of Physiology, Division of Nutrition Physiology, Federal University of São Paulo, São Paulo, Brazil
C. H. Koyama

¹Cancer Metabolism Research Group, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
E. C. Caperuto

³University of São Judas Tadeu, São Paulo, Brazil
M. L. Batista Jr

¹Cancer Metabolism Research Group, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
M.C. L. Seelaender

¹Cancer Metabolism Research Group, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil

Abstract

Bearing in mind that cancer cachexia is associated with chronic systemic inflammation and that endurance training has been adopted as a nonpharmacological anti-inflammatory strategy, we examined the effect of 8 weeks of moderate intensity exercise upon the balance of anti- and pro-inflammatory cytokines in 2 different depots of white adipose tissue in cachectic tumour-bearing (Walker-256 carcinosarcoma) rats. Animals were assigned to a sedentary control (SC), sedentary tumour-bearing (ST), sedentary pair-fed (SPF) or exercise control (EC), exercise tumour-bearing (ET), and exercise pair-fed (EPF) group. Trained rats ran on a treadmill (60% VO₂max) 60 min/day, 5 days/week, for 8 weeks. The retroperitoneal (RPAT) and mesenteric (MEAT) adipose pads were excised and the mRNA (RT-PCR) and protein (ELISA) expression of IL-1β, IL-6, TNF-α, and IL-10 were evaluated. The number of infiltrating monocytes in the adipose tissue was increased in cachectic rats. TNF-α mRNA in MEAT was increased in the cachectic animals (p<0.05) in relation to SC. RPAT protein expression of all studied cytokines was increased in cachectic animals in relation to SC and SPF (p<0.05). In this pad, IL-10/TNF-α ratio was reduced in the cachectic animals in comparison with SC (p<0.05) indicating inflammation. Exercise training improved IL-10/TNF-α ratio and induced a reduction of the infiltrating monocytes both in MEAT and RPAT (p<0.05), when compared with ST. We conclude that cachexia is associated with inflammation of white adipose tissue and that exercise training prevents this effect in the MEAT, and partially in RPAT.

Key words

cancer-cachexia - adipose tissue - cytokines - exercise training - inflammation

Full Text

References

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